ສຶກສາປະລິມານ Gymnemic Acid I ໃນຂົນຟືມ

Quantification of Gymnemic Acid I in Gymnema Sylvestre

Abstract

This study aims to analyze the concentration of Gymnemic acid I in Gymnema sylvestre leaves collected from 3 regions of 2 provinces: Tharat Village, Yommalat District, Khammouan Province (KM), PhonphapaoVillage, Sisattanak District (ITM) and Samsaard Village (Vernkham), Xaythany District (VK), Vientiane Capital. Three plant samples were collected by collecting leaves and shoots in July 2023. The leaves were washed with clean water, dried in the shade for 3 days, and baked at 60°C for 72 hours before being ground finely. The extraction was carried out by using EtOH : H₂O (2:1) solution, partitioned with Hexane, and then with CHCl₃ : EtOH (2:1). The 100 mg of the dried extract was dissolved in 25 mL of methanol, filtered through a 0.45 μm membrane filter, and analyzed with an HPLC. From direct sample analysis, it was found that the Gymnemic acid I in the samples VK and ITM was less than 0.27 mg/100 g DW and that of KM = 0.30 mg/100 g DW. By removing other substances first by passing through a chromatography column and TLC plate and then analyzing with HPLC, the Gymnemic acid I concentration in the samples VK = 2.5 mg/100 g DW, ITM = 2.9 mg/100g DW and KM = 4.2 mg/100g DW was found. The samples collected from the 3 areas had different Gymnemic acid I concentrations, which may be due to different environmental conditions and cultivation methods in each area.